Hi Welcome To My Page
MY RESEARCH AREA
Our lab is dedicated to understanding axon growth and regeneration, with a particular focus on why spinal cord neurons fail to regenerate after injury. By uncovering the molecular mechanisms behind axon regrowth, we aim to develop strategies that promote neural repair. Our research integrates multiple disciplines, including animal surgery, tissue culture, cloning, genomics, microscopy, and bioinformatics, allowing us to explore regeneration from a comprehensive, multi-scale perspective.
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BIOINFORMATICS
Bioinformatics plays a crucial role in our lab, enabling data mining, filtering, and identifying pro-growth genes to understand the molecular mechanisms driving neuroregeneration. It allows us to process large-scale genomic data, extract meaningful insights, and visualize complex biological relationships through graphs, heatmaps, and trajectory analyses.
We leverage computational tools such as R-Studio, TOBIAS, TF-COMB, Seurat, and trajectory analysis to analyze gene expression, chromatin accessibility, and transcription factor interactions. These tools help us uncover regulatory networks, identify key molecular players, and predict potential therapeutic targets.
By integrating bioinformatics into our research, we bridge the gap between raw sequencing data and biological discovery, facilitating new insights into neuronal repair and regeneration. Our approach helps drive data-driven neuroscience, bringing us closer to innovative solutions for paralysis and neurodegenerative diseases.
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CLONING
In my research, I utilize a diverse range of cloning techniques to design and engineer genetic constructs for AAV production, gene treatment, and gene manipulation. These methods allow for precise modifications of genetic sequences, enabling gene overexpression, knockdown, and targeted functional studies.
I commonly use PCR cloning, cut-and-paste cloning, Gibson assembly, and insertional & deletion mutagenesis to construct and optimize expression vectors tailored for specific experimental needs. These approaches help in generating plasmids that efficiently drive gene expression in various biological systems. Additionally, for gene silencing and targeted genome modifications, I employ CRISPR cloning for knockdown, which enables precise gene editing and regulatory control at the genomic level.
By integrating these advanced cloning strategies, I develop customized vectors suited for neuroscience research, allowing for the investigation of gene functions, neuroregeneration pathways, and potential therapeutic interventions in gene therapy. My work focuses on refining these genetic tools to better understand and manipulate molecular mechanisms underlying neural repair and regeneration.
INHOUSE VECTOR PRODUCTION
I specialize in optimizing in-house AAV production for gene therapy research, a critical domain that significantly saves time and reduces costs for the lab. This expertise has granted me the freedom to experiment with an unlimited number of gene treatments, enabling cutting-edge research in gene manipulation and neuroregeneration. To date, I have successfully produced over 40 unique AAVs, worth more than 3 crore, contributing to various experimental needs and advancing neuroscience projects.
In addition to AAV production, I possess strong expertise in generating Lentivirus vectors, essential tools for stable gene delivery and knockdown experiments. My experience also extends to the cloning of different cDNAs of transcription factors, which I have used extensively in my research to study gene regulation and functional outcomes. This comprehensive skill set not only supports the laboratory’s research goals but also allows for the creation of customized gene delivery systems that are crucial for the exploration of molecular mechanisms underlying neurological diseases.
With a deep understanding of viral vector production and cloning, I bring a unique and valuable skill set to optimize and accelerate experimental workflows, driving innovative breakthroughs in gene therapy and molecular biology.
TISSUE CULTURE
I have received extensive training in tissue culture techniques, equipping me with the expertise to handle various cell lines, including HEK293T, Neuro-2a (N2a), and primary neuronal cells. Each of these cell lines serves a distinct purpose in my research—HEK293T cells are widely used for AAV and Lentivirus production, Neuro-2a cells are instrumental in neurite outgrowth assays and drug screening, and primary neuronal cells provide a physiologically relevant system for studying neuronal development and regeneration.
I have successfully optimized high-throughput screening assays, including CRISPR oligo screening and N2a-based assays for identifying growth-promoting compounds, which are critical for therapeutic research. Additionally, I use tissue culture techniques to produce AAV and Lentivirus in bulk, ensuring high-efficiency viral vector generation for gene therapy applications.
With a strong foundation in cell-based assays, gene screening, and viral vector production, I bring a unique skill set that enhances experimental workflows and accelerates discoveries in neuroscience and regenerative medicine.
ADVANCE MICROSCOPY
I have extensive training in advanced microscopy techniques, specializing in high-resolution imaging for cellular and molecular research. My expertise includes the use of cutting-edge imaging systems such as LEICA STED, SP8 Confocal, Apotome, and Olympus Fluorescent Microscopes. These platforms enable precise visualization of subcellular structures, protein localization, and dynamic cellular processes with exceptional clarity.
In addition to imaging, I am highly skilled in tissue processing techniques, including tissue fixation, embedding, vibratome sectioning, and cryosectioning. I have optimized protocols to produce thin tissue sections ranging from 20 to 50 µm, essential for high-resolution imaging and histological analysis. These techniques are crucial for studying neural circuits, cellular morphology, and molecular interactions in neuroscience research.
By integrating advanced microscopy with tissue processing expertise, I offer a comprehensive approach to high-resolution imaging, sample preparation, and data-driven biological research, accelerating discoveries in neurobiology and regenerative medicine.
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GENOMICS
Genomics is one of the most advanced and powerful techniques in my skillset, allowing for in-depth analysis of gene expression, chromatin accessibility, and translational regulation. I have expertise in single-nucleus RNA sequencing (snRNA-seq) library preparation, enabling the study of gene expression at the single-cell level, as well as single-nucleus ATAC sequencing (snATAC-seq) library preparation, which provides insights into chromatin accessibility and transcription factor dynamics.
Additionally, I am proficient in Ribo-seq, a cutting-edge technique for studying active translation at the ribosome level, and quantitative PCR (qPCR) for precise gene expression analysis. My experience also extends to Fluorescence-Activated Cell Sorting (FACS), which I use to isolate specific cell populations for downstream genomic and transcriptomic analyses.
By combining these genomic and molecular techniques, I can generate high-quality datasets, uncover gene regulatory networks, and provide deep biological insights—all essential for advancing research in neuroscience, gene regulation, and regenerative medicine.
